Selection of W42 recombinant Proteins
Enzyme / Protein
| Productivity
| Source
| Comment
|
D-Amino acid oxidase
| 180 U/ml, app. 30 g/l
| Trigonopsis variabilis
|
|
Glutaryl-7-ACA acylase
| 180 U/ml, app. 30 g/l
| Pseudomonas sp.
|
|
Penicillin G acylase
| 29 U/ml, app. 10 g/l
| Achromobacter sp.
|
|
Penicillin G amidase
| 64 U/ml, app. 13 g/l
| E. coli
|
|
Penicillin G amidase
| 66 U/ml, app. 13 g/l
| Providencia rettgeri
|
|
Amino acylase I
| 232 U/ml, app. 5 g/l
| Porcine
|
|
a-Amino acid acetyl hydrolase
| 68 U/ml Ampicillin
260 U/ml Cefalexin
1.7 mU/ml D-phenylglycinemethyl ester
214 U/ml D-(p-hydroxy)phenyl glycinemethyl ester
| Xanthomonas maltophilia
|
|
D-Hydantoinase 1
| 458 U/ml Hydantoin
627 U/ml Phenylhydantoin
77 U/ml p-hydroxyphenylhyda.
app. 17 g/l
| Bacillus
thermoglucosidasius
|
|
D-Hydantoinase 2
| 449 U/ml Hydantoin
252 U/ml Phenylhydantoin
9 U/ml p-hydroxyphenylhydan.
app. 12 g/l
| Thermus sp.
|
|
D-Carbamoylase
| 12 U/ml, app. 8 g/l
| Burkholderia pickettii
|
|
Lipase type B
two variants
| CaL-B1: 14 kU/ml, 5,4 g/l
CaL-B2: 8 kU/ml, 4,9 g/l
Tributyrin assay
| Candida antarctica
|
|
Serum albumin
| 6,5 g/l
| Human
|
|
Protein A (modified)
three variants
| 3 - 6.5 g/l
| synthetic
|
|
Ceph C esterase
| 450 U/ml, app. 15 g/l
| Bacillus subtilis
|
|
Glutathion (GSH)
| 6 g/l
|
|
|
Lipase (RML)
|
| Rhizomucor miehei
| in progress
|
Lipase (TLL)
|
| Thermomyces lanuginosus
| in progress
|
|
|
|
|
Esterase (PLE)
| 11U/ml p-nitrophenylacetate
| pig liver
| in progress
|
Host organism is Pichia pastoris
intracellular expression = protein in culture volume in g/l
extracellular expresssion = protein in supernatant (cell free) in g/l
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