Selection of W42 recombinant Proteins

Enzyme / Protein

Productivity

Source

Comment

D-Amino acid oxidase
180 U/ml, app. 30 g/l
Trigonopsis variabilis

Glutaryl-7-ACA acylase
180 U/ml, app. 30 g/l
Pseudomonas sp.

Penicillin G acylase
29 U/ml, app. 10 g/l
Achromobacter sp.

Penicillin G amidase
64 U/ml, app. 13 g/l
E. coli

Penicillin G amidase
66 U/ml, app. 13 g/l
Providencia rettgeri

Amino acylase I
232 U/ml, app. 5 g/l
Porcine

a-Amino acid acetyl hydrolase
68 U/ml Ampicillin
260 U/ml Cefalexin
1.7 mU/ml D-phenylglycinemethyl ester
214 U/ml D-(p-hydroxy)phenyl glycinemethyl ester
Xanthomonas maltophilia

D-Hydantoinase 1
458 U/ml Hydantoin
627 U/ml Phenylhydantoin
77 U/ml p-hydroxyphenylhyda.
app. 17 g/l
Bacillus
thermoglucosidasius


D-Hydantoinase 2
449 U/ml Hydantoin
252 U/ml Phenylhydantoin
9 U/ml p-hydroxyphenylhydan.
app. 12 g/l
Thermus sp.

D-Carbamoylase
12 U/ml, app. 8 g/l
Burkholderia pickettii

Lipase type B
two variants

CaL-B1: 14 kU/ml, 5,4 g/l
CaL-B2:   8 kU/ml, 4,9 g/l

Tributyrin assay

Candida antarctica


Serum albumin
6,5 g/l
Human

Protein A (modified)
three variants

3 - 6.5 g/l

synthetic


Ceph C esterase
450 U/ml, app. 15 g/l
Bacillus subtilis


Glutathion (GSH)
6 g/l


Lipase (RML)

Rhizomucor miehei
in progress
Lipase (TLL)

Thermomyces lanuginosus
in progress




Esterase (PLE)
11U/ml p-nitrophenylacetate
pig liver
in progress

Host organism is Pichia pastoris  

intracellular expression = protein in culture volume in g/l

extracellular expresssion = protein in supernatant (cell free) in g/l 

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