Selection of W42 immobilized Enzymes


Biocatalyst

Activity / particle size

Assay

D-Amino acid oxidase
78 U/gsupply form 162 U/gdry, 100 - 300 µm
20 mM CephC, 1 bar O2
100 mM KiPO4, pH 8.0, 25°C
Glutaryl-7-ACA acylase
82 U/gsupply form 176 U/gdry
100-300 µm
20 g/l Gl-7-ACA, 5 mM KiPO4
pH 8.0 by NaOH, 37°C
Penicillin G amidase
Achromobacter sp.
217 U/gsupply form 717 U/gdry
100 - 300 µm

50 g/l PenG, 20 mM KiPO4
pH 8.0 by NaOH, 28°C
Penicillin G amidase
Achromobacter sp.
99 U/gsupply form, 289 U/gdry
200 - 500 µm
50 g/l PenG, 20 mM KiPO4
pH 8.0 by NaOH, 28°C

Penicillin G amidase
Escherichia coli
142 U/gsupply form, 333 U/gdry
100 - 300 µm
50 g/l PenG, 20 mM KiPO4
pH 8.0 by NaOH, 28°C

Penicillin G amidase WT
Providencia rettgeri
119 U/gsupply form, 372 U/gdry
200 - 300 µm
50 g/l PenG, 20 mM KiPO4
pH 8.0 by NaOH, 28°C
PenG amidase evolved
Providencia rettgeri
180 U/gsupply form, 581 U/gdry
100 - 300 µm
50 g/l PenG, 20 mM KiPO4
pH 8.0 by NaOH, 28°C
a-Amino acid acetyl
hydrolase

Xanthomonas maltophilia
107 U/gsupply form
200 - 500 µm
50 mM phenylglycine
methylester
10 mM KiPO4,
pH 6.0, 25°C
Amino acylase I porcine
(LAA)
101 U/gsupply form, 306 U/gdry
100 - 300 µm
50 mM N-AcMet, 100 mM KiBO3
pH 8.0, 37°C
N-Acetyl amino acid racemase
Deinococcus radiodurans
34 U/gsupply form, 126 U/gdry
100 - 300 µm
50 mM D-N-AcMet in Tris,
2.5 U/ml LAA, pH 8.0, 37°C
Cephalosporin C
Acetyl hydrolase
Bacillus subtilis
211 U/gsupply form, 622 U/gdry
100 - 300 µm
75 mM CephC, 10 mM KiPO4,
pH 8.0 by NaOH, 25°C
CaL-B enzymes (two var.)

all 100 - 300 µm

four different assays

Lipase CaL-B1
Lipase CaL-B2
17 kU/supply form, 23,5 kU/gdry
 9  kU/supply form, 12 kU/gdry

20 mM tributyrin, pH 7
25°C
Lipase CaL-B1
Lipase CaL-B2
2,3 kU/gdry   in progress
2,1 kU/gdry
20 mM myristic acid
25°C
Lipase CaL-B1
Lipase CaL-B2
17 kU/gdry   in progress
57 kU/gdry
1 mM p-nitrophenylbutyrate
pH 7; 25°C
Lipase CaL-B1
Lipase CaL-B2
635 U/gdry   in progress
468 U/gdry
2 mmol phenylethanol
20 mmol vinylacetate, 25°C
D-Hydantoinase (2 enzymes)

all 100 - 300 µm

three different substrates

Bacillus thermoglucosidasius
Thermus sp.
213 U/gsupply form, 539 U/gdry
615 U/gsupply form, 2.2 kU/gdry
80 mM hydantoin
pH 8.5, 37°C
Bacillus thermoglucosidasius
Thermus sp.
107 U/gsupply form, 344 U/gdry
390 U/gsupply form, 1.4 kU/gdry
80 mM phenylhydantoin
pH 8.5, 37°C
Bacillus thermoglucosidasius
Thermus sp.
13 U/gsupply form, 42 U/gdry
9.5 U/gsupplyform, 35 U/gdry
80 mM p-hydroxyphenylhyd.
pH 8.5, 37°C



D-Carbamoylase
Burkholderia pickettii
13.9 U/gsupply form, 49 U/gdry
100-300 µm
10 mM N-p-HPCG
50 mM KiPO4, pH 7.0 37°C
Trypsin bovine
(purchased)
9.5 U/gsupply form, 23 U/gdry
100 - 300 µm
2 mM BAPNA, 20 mM CaCl2
50 mM Tris, pH 7.6, 25°C

All enzymes/proteins are available in enriched solution or immobilized on special resins 

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