Biocatalyst
| Activity / particle size
| Assay
|
D-Amino acid oxidase
| 78 U/gsupply form 162 U/gdry, 100 - 300 µm
| 20 mM CephC, 1 bar O2
100 mM KiPO4, pH 8.0, 25°C
|
Glutaryl-7-ACA acylase
| 82 U/gsupply form 176 U/gdry
100-300 µm
| 20 g/l Gl-7-ACA, 5 mM KiPO4
pH 8.0 by NaOH, 37°C
|
Penicillin G amidase
Achromobacter sp.
| 217 U/gsupply form 717 U/gdry
100 - 300 µm
| 50 g/l PenG, 20 mM KiPO4
pH 8.0 by NaOH, 28°C
|
Penicillin G amidase
Achromobacter sp.
| 99 U/gsupply form, 289 U/gdry
200 - 500 µm
| 50 g/l PenG, 20 mM KiPO4
pH 8.0 by NaOH, 28°C
|
Penicillin G amidase
Escherichia coli
| 142 U/gsupply form, 333 U/gdry
100 - 300 µm
| 50 g/l PenG, 20 mM KiPO4
pH 8.0 by NaOH, 28°C
|
Penicillin G amidase WT
Providencia rettgeri
| 119 U/gsupply form, 372 U/gdry
200 - 300 µm
| 50 g/l PenG, 20 mM KiPO4
pH 8.0 by NaOH, 28°C
|
PenG amidase evolved
Providencia rettgeri
| 180 U/gsupply form, 581 U/gdry
100 - 300 µm
| 50 g/l PenG, 20 mM KiPO4
pH 8.0 by NaOH, 28°C
|
a-Amino acid acetyl
hydrolase
Xanthomonas maltophilia
| 107 U/gsupply form
200 - 500 µm
| 50 mM phenylglycine
methylester 10 mM KiPO4,
pH 6.0, 25°C
|
Amino acylase I porcine
(LAA)
| 101 U/gsupply form, 306 U/gdry
100 - 300 µm
| 50 mM N-AcMet, 100 mM KiBO3
pH 8.0, 37°C
|
N-Acetyl amino acid racemase
Deinococcus radiodurans
| 34 U/gsupply form, 126 U/gdry
100 - 300 µm
| 50 mM D-N-AcMet in Tris,
2.5 U/ml LAA, pH 8.0, 37°C
|
Cephalosporin C
Acetyl hydrolase
Bacillus subtilis
| 211 U/gsupply form, 622 U/gdry
100 - 300 µm
| 75 mM CephC, 10 mM KiPO4,
pH 8.0 by NaOH, 25°C
|
CaL-B enzymes (two var.)
| all 100 - 300 µm
| four different assays
|
Lipase CaL-B1
Lipase CaL-B2
| 17 kU/supply form, 23,5 kU/gdry
9 kU/supply form, 12 kU/gdry
| 20 mM tributyrin, pH 7
25°C
|
Lipase CaL-B1
Lipase CaL-B2 | 2,3 kU/gdry in progress
2,1 kU/gdry
| 20 mM myristic acid
25°C
|
Lipase CaL-B1
Lipase CaL-B2 | 17 kU/gdry in progress
57 kU/gdry
| 1 mM p-nitrophenylbutyrate
pH 7; 25°C
|
Lipase CaL-B1
Lipase CaL-B2 | 635 U/gdry in progress
468 U/gdry
| 2 mmol phenylethanol
20 mmol vinylacetate, 25°C
|
D-Hydantoinase (2 enzymes)
| all 100 - 300 µm
| three different substrates
|
Bacillus thermoglucosidasius
Thermus sp.
| 213 U/gsupply form, 539 U/gdry
615 U/gsupply form, 2.2 kU/gdry
| 80 mM hydantoin
pH 8.5, 37°C
|
Bacillus thermoglucosidasius
Thermus sp.
| 107 U/gsupply form, 344 U/gdry
390 U/gsupply form, 1.4 kU/gdry
| 80 mM phenylhydantoin
pH 8.5, 37°C
|
Bacillus thermoglucosidasius
Thermus sp.
| 13 U/gsupply form, 42 U/gdry
9.5 U/gsupplyform, 35 U/gdry
| 80 mM p-hydroxyphenylhyd.
pH 8.5, 37°C
|
|
|
|
D-Carbamoylase
Burkholderia pickettii
| 13.9 U/gsupply form, 49 U/gdry
100-300 µm
| 10 mM N-p-HPCG
50 mM KiPO4, pH 7.0 37°C
|
Trypsin bovine
(purchased)
| 9.5 U/gsupply form, 23 U/gdry
100 - 300 µm
| 2 mM BAPNA, 20 mM CaCl2
50 mM Tris, pH 7.6, 25°C
|